Malignancies – lymphoma, chronic lymphocytic leukemia (rare in both)
Clinical Presentation
Similar to pemphigoid or epidermolysis bullosa acquisita
Papulovesicles, bullae or urticarial plaques on extensor, central or flexural sites with truncal involvement
Involvement of oral mucosal is common in adult disease, ocular involvement also occurs
May have severe pruritus
“String of pearls” lesion consisting of grouped vesicles
Perineal and perioral involvement common in children
Pathology and Immunopathology
IgA at the basement membrane zone in perilesional tissue by direct immunofluorescence; complement and lesser intense staining of IgG and IgM may also be found
Neutrophil involvement in linear IgA disease is usually greater than in pemphigoid and epidermolysis bullosa acquisita in which eosinophil involvement is prominent
Serum IgA basement membrane zone antibodies by indirect immunofluorescence binding to the epidermal, dermal or combined epidermal-dermal areas of split skin.
97 kD LABD and LAD-1 are cleavage products of BP180 and are major antigenic targets
Type VII collagen may be antigen accounting for dermal staining on split skin
Diagnosis
Laboratory testing
Perilesional skin biopsy
IgA basement membrane zone antibodies positive in 80-90%
Serum
Antibodies localized to the epidermal side of split skin or showing a combined epidermal and dermal pattern
Tests generally appear in the order most useful for common clinical situations
Click on number for test-specific information in the ARUP Laboratory Test Directory
Test Name and Number
Recommended Use
Limitations
Follow Up
Epithelial Basement Membrane Zone IgA Antibodies 0092057
Method: Indirect Immunofluorescence
Discriminate among clinically similar immune-mediated skin diseases such as linear IgA disease, pemphigoid, epidermolysis bullosa acquisita and dermatitis herpetiformis in patients suspected of having or known to have any type of subepidermal immunobullous disease
This test is specific for IgA BMZ antibodies found in linear IgA disease and will not detect IgG BMZ antibodies found in pemphigoid or epidermolysis bullosa acquisita
Perilesional skin biopsy may be necessary for final clinical diagnosis
General References
Allen J, Wojnarowska F.Linear IgA disease: the IgA and IgG response to the epidermal antigens demonstrates that intermolecular epitope spreading is associated with IgA rather than IgG antibodies, and is more common in adults.Br J Dermatol. 2003;149(5):977-985. (Link to PubMed)
Harman KE.New laboratory techniques for the assessment of acquired immunobullous disorders.Clin Exp Dermatol. 2002;27(1):40-46. (Link to PubMed)
Mutasim DF, Adams BB.Immunofluorescence in dermatology.J Am Acad Dermatol. 2001;45(6):803-822. (Link to PubMed)
Zone JJ, Taylor TB, Meyer LJ, Petersen MJ.The 97 kDa linear IgA bullous disease antigen is identical to a portion of the extracellular domain of the 180 kDa bullous pemphigoid antigen, BPAg2.J Invest Dermatol. 1998;110(3):207-210. (Link to PubMed)
Reviewed by
Leiferman, Kristin M., M.D. Co-Director, Immunodermatology Laboratory at ARUP Laboratories; Professor, Dermatology, University of Utah
Zone, John J., M.D. Co-Director, Immunodermatology Laboratory at ARUP Laboratories; Professor and Chairman, Dermatology, University of Utah
Comprehensive Review: September 2007
Last Update: September 2007