Human leptospirosis is an acute febrile illness that presents with many manifestations and is found worldwide.
Epidemiology
Transmission
Leptospirosis is a zoonotic disease of worldwide prevalence caused by the spirochete Leptospira interrogans
Although wild mammals (e.g., rodents) serve as a primary natural reservoir, domestic animals (dogs, cattle, swine, horses) serve as a major source of human infection
Approximately one-half of infections occur from July to October, but cases can occur throughout the year
Most often transmission is indirect, by human contact with soil, food or water contaminated by urine from an infected animal
Transmission of organism to humans has occurred through contaminated well water, spring water, food-preparation surfaces and by swimming, rafting or kayaking in water sources where livestock have been pastured
Certain occupational groups (agriculture, sewer, construction and stock handling) have been found to be at particular risk for leptospirosis
Organism
Spirochete
Member of the family Spirochaetales which also includes Treponema and Borrelia
Risk Factors
Contaminated water
Poor sanitation
Clinical Presentation
Leptospiremia follows after an 8-12 day incubation period, with a variable clinical course
Infection may present:
As subclinical and only detectable by serologic means
With an influenza-like febrile illness with high fever, chills, rigor, myalgia, abdominal pain, vomiting and diarrhea
As severe, life-threatening multi-organ failure
Jaundice occurs infrequently in the U.S.; leptospiral meningitis occurs more frequently, accounting for 5-13% of sporadic lymphocytic meningitis cases
Kidneys are invariably involved; urine analysis demonstrates leukocytes, erythrocytes, casts and proteinuria
Diagnosis
Laboratory Testing
Leptospira antibody testing
The laboratory diagnosis of leptospirosis is complicated by the fact that the organism is fastidious and slow-growing, occasionally requiring weeks to grow; in this context, serology represents an acceptable alternative that may be more immediate
Differential Diagnosis
Differential diagnosis includes dengue, malaria, enteric fever, Hantavirus and rickettsial disease
Treatment
Treat with antimicrobials
Jarisch-Herxheimer reaction (fever, myalgias, headache, tachycardia, hypotension) may occur with initiation of treatment for all spirochetal diseases
Detect the presence of serum IgM to Leptospira biflexa, serovar patoc 1, which is genetically similar to disease-causing members of the genus Leptospira
Detect Leptospira-specific IgM in serum or plasma samples in which a clinical suspicion of Leptospira infection exists
Equivocal specimens should be cautiously interpreted; further testing with an additional specimen is recommended
If the specimen remains equivocal, a second serological method should be considered if leptospirosis infection is still suspected
Detect Leptospira-specific IgG and IgM in serum or plasma samples in patients for whom Leptospira infection is suspected
This assay cannot be used to predict the point of IgM appearance or cessation in patients, nor can it be used to distinguish a current or recent leptospirosis infection from a past infection or reinfection with another serotype
Test is less sensitive than IgM by Dot Blot
If the results remain equivocal after retesting, a second serological method should be considered if leptospirosis infection is still suspected
Equivocal specimens should be cautiously interpreted; further testing with an additional specimen is recommended
Method: Standard reference procedures for Leptospira bacterial culture and exam
Gold standard for Leptospira identification
Time sensitive test
Additional Information
An initially negative result followed by a positive result indicates IgM seroconversion in Dot Blot test.
General References
Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM.Leptospirosis: a zoonotic disease of global importance.Lancet Infect Dis. 2003;3(12):757-771. (Link to PubMed)
Carvalho CR, Bethlem EP.Pulmonary complications of leptospirosis.Clin Chest Med. 2002;23(2):469-478. (Link to PubMed)
Edwards CN, Levett PN.Prevention and treatment of leptospirosis.Expert Rev Anti Infect Ther. 2004;2(2):293-298. (Link to PubMed)
Higgins R.Emerging or re-emerging bacterial zoonotic diseases: bartonellosis, leptospirosis, Lyme borreliosis, plague.Rev Sci Tech. 2004;23(2):569-581. (Link to PubMed)
Levett PN.Leptospirosis.Clin Microbiol Rev. 2001;14(2):296-326. (Link to PubMed)
McBride AJ, Athanazio DA, Reis MG, Ko AI.Leptospirosis.Curr Opin Infect Dis. 2005;18(5):376-386. (Link to PubMed)
Reviewed by
Litwin, Christine, M.D. Medical Director, Immunology at ARUP Laboratories; Professor, Clinical Pathology, University of Utah
Petti, Cathy A., M.D. Medical Director, Infectious Diseases at ARUP Laboratories; Assistant Professor, Pathology and Medicine, University of Utah
Comprehensive Review: November 2007
Last Update: November 2007