Preferred test in most clinical scenarios; best test for rheumatic chorea since it remains elevated longer
Confirm current or recent group A streptococcal infection in patients suspected of having a non-suppurative complication such as acute glomerulonephritis (AGN) or acute rheumatic fever (ARF)
DNase-B and ASO antibody tests are generally ordered concurrently
Antibiotic therapy decreases antibody levels
May want to order a throat culture at the same time
Recommend testing acute and convalescent samples taken approximately 2 weeks apart
Antibodies may persist for 2-3 months after infection
Confirm a prior infection with group A streptococcus in patients suspected of having a non-suppurative complication, such as acute glomerulonephritis or acute rheumatic fever
DNase-B and ASO antibody tests are generally ordered concurrently
Not helpful in the diagnosis of the agent of skin infections
Antibiotic therapy decreases elevation of test
May want to order a throat culture at the same time
Recommend testing acute and convalescent samples taken approximately 2 weeks apart
Antibodies may persist for 6 weeks after infection
Streptococcus pyogenes, Group A Antibody (Streptozyme®) with Reflex to Titer 0050642
Method: Hemagglutination
Outdated test
Recommend DNase-B Antibody or Streptolysin O Antibody tests
Additional Tests Available
Click on number for test-specific information in the ARUP Laboratory Test Directory
Method: Standard reference procedures for aerobic bacterial culture and identification
Streptococcus (Group A) Rapid with Reflex to Culture 0028903
Method: Immunoassay with Direct Observation/Culture
Additional Information
Antibody testing
Fourfold increase in titers between acute and convalescent samples taken approximately 2 weeks apart are confirmatory of recent or current infection with group A streptococci.
If antibody test results are equivocal, consider retesting in 10-14 days.
General References
Bisno AL.Acute pharyngitis.N Engl J Med. 2001;344(3):205-211. (Link to PubMed)
Bourbeau PP.Role of the microbiology laboratory in diagnosis and management of pharyngitis.J Clin Microbiol. 2003;41(8):3467-3472. (Link to PubMed)
Colletti T, Robinson P.Strep throat: guidelines for diagnosis and treatment.JAAPA. 2005;18(9):38-44. (Link to PubMed)
Hahn RG, Knox LM, Forman TA.Evaluation of poststreptococcal illness.Am Fam Physician. 2005;71(10):1949-1954. (Link to PubMed)
Shet A, Kaplan EL.Clinical use and interpretation of group A streptococcal antibody tests: a practical approach for the pediatrician or primary care physician.Pediatr Infect Dis J. 2002;21(5):420-426. (Link to PubMed)
References from the ARUP Institute for Clinical and Experimental Pathology®
Martins TB, Augustine NH, Hill HR.Development of a multiplexed fluorescent immunoassay for the quantitation of antibody responses to group A streptococci.J Immunol Methods. 2006;316(1-2):97-106. (Link to PubMed)
Martins TB, Veasy LG, Hill HR.Antibody responses to group A streptococcal infections in acute rheumatic fever.Pediatr Infect Dis J. 2006;25(9):832-837. (Link to PubMed)
Reviewed by
Hill, Harry R., M.D. Group Medical Director, Laboratory of Immunology, ARUP Laboratories, and Executive Director of the ARUP Institute for Clinical and Experimental Pathology; Professor and Division Head, Clinical Pathology, University of Utah
Litwin, Christine, M.D. Medical Director, Immunology at ARUP Laboratories; Professor, Clinical Pathology, University of Utah
Comprehensive Review: September 2007
Last Update: September 2007