| Test Name and Number | Recommended Use | Limitations | Follow Up |
|---|
| Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture 0060280 Method: |
Traditionally, gold standard test for identifying acute HSV infection in acute lesions (e.g., vesicles, ulcers, inflammation of mucous membranes)
| |
|
| Herpes Simplex Virus Culture 0065005 Method: Cell Culture/Immunoassay
|
Traditionally, gold standard test for identifying acute HSV infection in acute lesions (e.g., vesicles, ulcers, inflammation of mucous membranes)
Standard of care for diagnosing HSV meningitis/meningoencephalitis in CSF specimens
| |
May want to order varicella-zoster virus and herpes simplex virus DFA with reflex to varicella-zoster virus culture to clarify etiology
|
| Herpes Simplex Virus by PCR 0060041 Method: Polymerase Chain Reaction
|
Rapid diagnostic for:
- CSF in herpes encephalitis
- CSF in unusual CNS syndromes and in AIDS patients
- Patients with recurrent meningitis
- Infants with suspected neonatal herpes where cultures have been negative (e.g., CSF nasopharyngeal aspirate)
Resolve etiology of lesions several days old in situations where cultures are more likely to be negative
Standard of care for diagnosing HSV meningitis/meningoencephalitis in CSF specimens
| A negative result does not rule out PCR inhibitors in patient specimen or herpes simplex virus DNA concentrations below level assay can detect |
|
| Herpes Simplex Virus Culture with Reflex to HSV Typing 0065065 Method: Cell Culture/Immunoassay
|
Detect herpes simplex virus and confirm HSV as type 1 or 2
Standard of care for diagnosing HSV meningitis/meningoencephalitis in CSF specimens
| Herpes simplex virus by PCR on CSF specimens is a more sensitive method for detection of herpes simplex virus encephalitis |
|
| Herpes Simplex Virus Type 1 & Type 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot (HerpeSelect®) 0050459 Method: Immunoblot
|
Differentiate between HSV types 1 and 2
| Individuals infected with HSV may not exhibit detectable IgG antibody to glycoprotein G in the early stages of infection and 5-10% of infections may occur with glycoprotein G-deficient virus |
|
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG & IgM 0050291 Method: Enzyme-Linked Immunosorbent Assay/Chemiluminescent Immunoassay
|
Diagnose acute infection
Note: acute and convalescent samples required for proper interpretation, even with tests for IgM antibody, as low levels of IgM antibody may persist months after initial infection
| HSV-specific types may not be identified because some patients may not develop antibodies to Glycoprotein-G Low levels of IgM antibody may persist months after initial infection |
|
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG with Reflex to Type 1 & 2 Glycoprotein G-Specific Ab, IgG 0051708 Method: Refer to individual components.
|
Detect antibodies to HSV
Differentiate between types 1 and 2
| | |
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG & IgM with Reflex to Type 1 & 2 Glycoprotein G-Specific Ab, IgG 0050916 Method: Refer to individual components
|
Identify type of HSV in a patient who is exhibiting symptoms or in a patient who has recent exposure but has yet to show symptoms
| Low levels of IgM antibody may persist months after initial infection |
|
| Herpes Simplex Type 1 & Type 2 Glycoprotein G-Specific Antibodies, IgG by ELISA (HerpeSelect®) 0051152 Method: Enzyme-Linked Immunosorbent Assay
|
Detect antibodies to HSV
Differentiate between types 1 and 2
| |
|
| Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) 0050294 Method: Enzyme-Linked Immunosorbent Assay
|
Differentiate between HSV types 1 and 2
| A few patients will not form glycoprotein so testing will be negative |
|
| Herpes Simplex Virus Type 1 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) 0050292 Method: Enzyme-Linked Immunosorbent Assay
|
Differentiate between HSV types 1 and 2
| A few patients will not form glycoprotein so testing will be negative |
|
| Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®), CSF 0050359 Method: Enzyme-Linked Immunosorbent Assay
|
Identify the type of HSV in a patient with meningitis
| |
|
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG & IgM (CSF) with Reflex to Type 1 & 2 Glycoprotein G-Specific Ab, IgG 0050364 Method: Refer to individual components
|
Identify the type of HSV in a patient with meningitis
Detect antibodies to HSV in cerebral spinal fluid (CSF)
| Low levels of IgM antibody may persist months after initial infection |
|
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG, CSF 0050394 Method: Chemiluminescent Immunoassay
|
Identify from CNS specimens seropositive organ transplant recipients who often have prophylactic antiviral therapy initiated prior to the transplantation procedure
| |
|
| Immunohistochemistry Stain Offering arup005 Method: Immunohistochemistry
|
For fixed tissue samples, consultative services as well as immunohistochemical staining for the presence of HSV 1 and HSV 2 are available
| | |