T-cell and NK-cell non-Hodgkin lymphomas (NHL) represent a small portion of the lymphomas diagnosed in the U.S.
Epidemiology
WHO Classification of Mature T- and NK-cell Neoplasms (2008)
Risk Factors
Clinical Presentation of Selected Lymphoma Subtypes (based on WHO classification)
| Test Name and Number | Recommended Use | Limitations | Follow Up |
|---|---|---|---|
| CBC with Platelet Count and Automated Differential 0040003 Method: Automated Cell Count/Differential |
Initial test in evaluation of lymphoma |
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| Hepatic Function Panel 0020416 Method: Quantitative Enzymatic/Quantitative Spectrophotometry |
Initial test in evaluation of lymphoma May provide prognostic information |
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| Lactate Dehydrogenase, Serum or Plasma 0020006 Method: Quantitative Enzymatic |
Initial test in evaluation of lymphoma May provide prognostic information |
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| Uric Acid, Serum or Plasma 0020026 Method: Quantitative Spectrophotometry |
Initial test in evaluation of lymphoma May provide prognostic information |
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| Leukemia/Lymphoma Phenotyping (Comprehensive - Whole Blood) 0096299 Method: Flow Cytometry |
Use for whole blood specimens to identify tumor cell populations, aid in selection of appropriate therapy and assessment of clinical behavior and prognosis T-cell antigens include CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, cytoplasmic CD3, CD26 Other antigens include CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2 |
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| Leukemia/Lymphoma Phenotyping (Comprehensive - Bone Marrow) 0095244 Method: Flow Cytometry |
Use for bone marrow specimens to identify neoplastic populations, aid in selection of appropriate therapy and assessment of clinical behavior and prognosis T-cell antigens include CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, cytoplasmic CD3 Other antigens include CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2 |
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| Leukemia/Lymphoma Phenotyping (Comprehensive - Miscellaneous) 0095243 Method: Flow Cytometry |
Use for fluid and tissues to identify tumor cell populations, aid in selection of appropriate therapy and assessment of clinical behavior and prognosis T-cell antigens include CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, cytoplasmic CD3 B-cell antigens include CD10, CD19, CD20, CD22, CD23, CD103, kappa, lambda, FMC7, cytoplasmic kappa, cytoplasmic lambda Myelo/mono antigens include CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD15, CD33, CD64, CD117, myeloperoxidase Other antigens include CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2 |
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| T-Cell Clonality by Flow Cytometry Analysis of TCR V-Beta 0093199 Method: Flow Cytometry |
Aid in further characterization of phenotypically abnormal T-cell populations identified by flow cytometry and determine whether monoclonality is present based on expression of T-cell antigen receptor beta chain variable regions (TCR V-Beta) |
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| T-Cell Clonality Screening by PCR 0055567 Method: Polymerase Chain Reaction/Capillary Electrophoresis |
Aid in identification and diagnosis of T-cell lymphomas |
"Not detected" result does not entirely exclude the presence of a T-cell receptor gamma rearrangement (or monoclonal T-cell population) in the sample "Not detected" PCR screening result should be terminally analyzed by restriction fragment Southern blot hybridization (RF-SBH) to definitively exclude T-cell monoclonality |
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| T-Cell Clonality Assessment by Restriction Fragment-Southern Blot Hybridization 0055596 Method: Restriction Fragment Southern Blot Hybridization |
Aid in identification and diagnosis of T-cell lymphomas Definitely confirms negative PCR T-cell clonality |
Negative result does not entirely exclude the presence of a T-cell receptor gene rearrangement (or monoclonal T-cell population) in the sample |
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| Human T-Lymphotropic Virus Types I/II Antibodies with Reflex to HTLV I/II Confirmation 0051164 Method: Qualitative Enzyme Immunoassay/Qualitative Western Blot |
Aid in diagnosis of adult T-cell leukemia/lymphoma |
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| Epstein-Barr Virus Antibody to Early D Antigen (EA-D), IgG 0050225 Method: Semi-Quantitative Chemiluminescent Immunoassay |
Aid in diagnosis of NK-cell nasal-type lymphomas |
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| CD2 by Immunohistochemistry 2003505 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD1a by Immunohistochemistry 2003502 Method: Immunohistochemistry |
Aid in identifying some T-cell lymphoblastic lymphomas and leukemias Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD3 by Immunohistochemistry 2003508 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD4 by Immunohistochemistry 2003511 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD5 by Immunohistochemistry 2003514 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD7 by Immunohistochemistry 2003517 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD8 by Immunohistochemistry 2003520 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD10 (CALLA) by Immunohistochemistry 2003523 Method: Immunohistochemistry |
Aid in identifying lymphoblastic lymphoma, Burkitt lymphoma, follicular lymphoma and CML; aids in differential diagnosis of small B-cell lymphomas and subtyping of lymphoblastic leukemias; helpful in angioimmunoblastic T-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD15, Leu M1 by Immunohistochemistry 2003529 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD19 by Immunohistochemistry 2005114 Method: Immunohistochemistry |
Aid in histologic differential diagnosis of T/NK-cell leukemia/lymphoma Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD21 (Dendritic Cell) by Immunohistochemistry 2003535 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD23 by Immunohistochemistry 2003541 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD25 by Immunohistochemistry 2003544 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD30 (Ki-1) by Immunohistochemistry 2003547 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD34, QBEnd/10 by Immunohistochemistry 2003556 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD43, L60 (Leu 22) by Immunohistochemistry 2003568 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD56 (NCAM) by Immunohistochemistry 2003589 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| CD57 by Immunohistochemistry 2003592 Method: Immunohistochemistry |
Aid in identifying NK lymphocyte-mediated cytotoxicity, NK and T-cell subset tumors and neural tissue neoplasms Stained and returned to client pathologist for interpretation; consultation available if needed |
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| Ki-67, MIB-1, by Immunohistochemistry 2004519 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| Anaplastic Lymphoma Kinase 1 (ALK-1) by Immunohistochemistry 2003439 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| BF-1 by Immunohistochemistry 2003466 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| Muc-1 by Immunohistochemistry 2004002 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |
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| T-cell Intracytoplasmic Antigen (TIA-1) by Immunohistochemistry 2004148 Method: Immunohistochemistry |
Aid in differentiating T-cell leukemias and lymphomas from B-cell leukemias and lymphomas |
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| TdT by Immunohistochemistry 2004142 Method: Immunohistochemistry |
Aid in histologic diagnosis of T/NK-cell lymphomas Stained and returned to client pathologist for interpretation; consultation available if needed |