| Test Name and Number | Recommended Use | Limitations | Follow Up |
|---|
| Protein Electrophoresis, Serum 0050640 Method: Capillary Electrophoresis
|
SPEP is the recommended test for patients with suspected plasma cell dyscrasia
SPEP can be used to monitor treatment response when plasma cell dyscrasia is known
| SPEP can be normal in patients with oligo-secretory on non-secretory myeloma |
Order: Immunofixation Electrophoresis (Serum), Quantitation IgA, IgG & IgM (Serum) 0050615
|
| Immunofixation Electrophoresis Monoclonal Protein Detection Quantitation & Characterization SPE, IFE, IgA, IgG, IgM 0050615 Method: Immunofixation Electrophoresis/Capillary Electrophoresis/Nephelometry
|
Identify and characterize the presence of the M protein or monoclonal free light chain components in patients with abnormal banding patterns from SPEP
IFE is more sensitive than SPEP in detecting M proteins
Monitor therapy and remission of disease
| IFE can be normal in patients with non-secretory myeloma |
Order: Bence-Jones Protein Qualitative Free Kappa & Lambda Light Chains (Urine) 0050161, skeletal survey and a bone marrow biopsy to rule out plasma cell dyscrasia if M protein detected as well as calcium and Beta-2 microglobulin concentration
|
| Protein Electrophoresis with Reflex to Immunofixation Electrophoresis Monoclonal Protein Detection, Quantitation & Characterization, IgA, IgG, & IgM, Serum 0050658 Method: Capillary Electrophoresis/Immunofixation Electrophoresis/Nephelometry
|
Distinguish between proteinuria due to renal disease and monoclonal light chains in serum for patient with renal disease
| SPEP can be normal in patients with oligo-secretory on non-secretory myeloma |
Order: Bence-Jones Protein Qualitative Free Kappa & Lambda Light Chains (Urine) 0050161, skeletal survey and a bone marrow biopsy to rule out plasma cell dyscrasia if M protein detected
|
| Bence Jones Protein, Qualitative Free Kappa & Lambda Light Chains, Urine 0050161 Method: Immunofixation Electrophoresis/Nephelometry
|
Diagnosis of Bence-Jones protein and its specificity
| |
|
| Kappa/Lambda Quantitative Free Light Chains with Ratio, Serum 0055167 Method: Nephelometry
|
Quantify kappa and lambda light chains in human serum
Diagnosis and monitoring of patients with oligo-secretory or non-secretory myeloma and light-chain amyloidosis
| Low levels of FLC are found in serum of normal individuals due to the over production and secretion of FLC by plasma cells |
Order sequential levels for monitoring disease progress to therapy
|
| Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine 0050618 Method: Immunofixation Electrophoresis/Nephelometry
|
Diagnosis and monitoring the presence of Bence-Jones protein and its specificity
| |
Sequential levels for monitoring disease progress
|
| Lambda Light Chain Immunoglobulin mRNA by in situ Hybridization 8033744 Method: in situ Hybridization
|
Identification of monoclonal plasma cell populations in tissue and bone marrow biopsies
| |
Useful in initial diagnosis and follow-up biopsies
|
| Immunofixation Electrophoresis, Immunoglobulin D & Immunoglobulin E, Serum 0050049 Method: Immunofixation Electrophoresis
|
Identify presence of monoclonal IgD or IgE gammopathy in patients with free kappa or lambda identified by IFE electrophoresis
| |
|
| Bence Jones Protein, Quantitative Free Kappa Light Chains, Urine 0050689 Method: Nephelometry/Immunofixation Electrophoresis
|
Monitor treatment response when gammopathy is known to be kappa
| |
Sequential levels for monitoring disease progress
|
| Bence Jones Protein, Quantitative Free Lambda Light Chains, Urine 0050682 Method: Nephelometry/Immunofixation Electrophoresis
|
Monitor treatment response when gammopathy is known to be lambda
| |
Sequential levels for monitoring disease progress
|
| Immunofixation Electrophoresis Gel 0050272 Method: Immunofixation Electrophoresis
|
Identify and characterize the presence of M protein
| Serum IFE does not provide quantification of M protein |
|
| Beta-2 Microglobulin, Serum or Plasma 0080053 Method: Immunoturbidimetric
|
Prognostic indicator for multiple myeloma
| |
|
| Chromosome Analysis, Bone Marrow 0097605 Method: Giemsa-Band Analysis
|
Detect chromosome abnormalities in bone marrow aspirate consistent with diagnosis of multiple myeloma
Some abnormalities also have prognostic significance
| Normal metaphase results are suggestive of a stroma-dependant early myeloma, whereas abnormal metaphase results are suggestive of a stroma-independent later-stage myeloma with an associated poorer prognosis. It is recommended this test be done in conjunction with multiple myeloma FISH panel for increased sensitivity, especially in early stage stroma-dependant myeloma |
Repeat testing as clinically indicated to monitor disease progression
|
| Chromosome Analysis, Multiple Myeloma Panel by FISH 0092617 Method: Fluorescence in situ Hybridization
|
Detect prognostically significant genomic aberrations in IGH/CCNDI, D13S25, IgH and p53 in non-dividing cells from bone marrow aspirate
If sufficient sample is available, a sorting method is used to select for plasma cells, increasing the sensitivity of assay
| FISH will only detect aberrations specific to probes utilized Test is to be used in conjunction with bone marrow chromosome analysis which may detect additional diagnostically significant chromosome abnormalities |
Repeat testing as clinically indicated to monitor disease progression
|
| Viscosity, Serum 0020056 Method: Cone-Plate Viscometer
|
Evaluation of hyperviscosity syndrome associated with plasma cell dyscrasia
| Patients with rheumatoid arthritis, lupus erythematosus or hyperfibrinogenemia occasionally may have increased serum viscosity in serum samples |
Repeat testing as clinically indicated to monitor disease progression
|
| Viscosity, Whole Blood 0020054 Method: Cone-Plate Viscometer
|
Evaluation of hyperviscosity syndrome associated with plasma cell dyscrasia
| Patients with rheumatoid arthritis, lupus erythematosus or hyperfibrinogenemia occasionally may have increased blood viscosity in serum samples |
Repeat testing as clinically indicated to monitor disease progression
|
| Immunohistochemistry Stain Offering arup005 Method: Immunohistochemistry
|
For fixed tissue samples, consultative cervices as well as immunohistochemical staining for CD52 (Campath), CD79a, CD138 (Syndican-1), IgA, IgD, IgG, IgM, Ki-67 (Mib-1), p53, plasma cell, kappa and lambda are available
| |
Useful in initial diagnosis as well as for follow-up of therapy
|