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Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgG and IgM by Immunoblot (Standard Two-Tier Testing)

LYME STTT
Example Reports
Negative
Positive

Ordering Recommendation

Reflex test to diagnose Lyme disease in symptomatic individuals. For use ≤4 weeks after symptom onset. Reflex pattern follows the standard two-tier testing (STTT) approach; a positive or equivocal screen is confirmed by immunoblot.

The preferred reflex test is Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing) (3006053).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Transfer 2 mL serum to an ARUP standard transport tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated:10 days; Frozen: 1 month.

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)/Qualitative Immunoblot

Performed

Sun-Sat

Reported

1-4 days

Reference Interval

Test Number
 Components
 Reference Interval
  B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less

Interpretive Data




Component Interpretation
B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less: Negative; VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV: Equivocal; repeat testing in 10-14 days may be helpful.
1.10 IV or greater: Positive; VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Compliance Category

FDA

Note

If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then B. burgdorferi IgG antibody by immunoblot and IgM antibody by immunoblot will be added. Additional charges apply.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/20/2024
X
N/A

CPT Codes

86618; if reflexed, add 86617 x2

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Components

Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 100711-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Lyme Disease Abs Reflex Panel
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgG and IgM by Immunoblot (Standard Two-Tier Testing)